MultispeQ Handheld Fluorometer
$125.00 w/o CO2
$190.00 w/ CO2
View the MultispeQ tutorials here!
PhotosynQ is a platform where anyone, anywhere can easily create, share, and analyze plant and soil health information using high quality scientific instruments.
The MultispeQ is the first device on the PhotosynQ platform and is capable of measuring leaf chlorophyll content, photosystem II efficiency, photosystem I activity, non-photochemical quenching, and the proton motive force, as well as soil measurements including soil biological activity and soil active carbon (humus). The PhotosynQ platform, and MultispeQ device, are completely open source (hardware, software, and data), so users can easily add and modify the system to their needs. PhotosynQ has applications for agricultural extension agents, plant breeders, citizen science and environmental activists, science educators, and of course plant scientists.
We believe that phenomic plant data, like genomic data, is a critical global resource and must both be shared and agglomerated to be useful.
Devices will automatically sync all user data to the cloud via users’ cell phones, where anyone can see and analyze it. In this way we can create a high quality, open set of plant and soil health data taken from around the world. Most importantly, the data is taken using the same instrument and protocols, making it highly comparable and consistent.
Within the Photosynq community you can create “experiments” via the website.
Each experiment contains directions to collaborators collecting data, which measurements will be taken, the location of the measurement, and other specifications of those collecting the data in the field. Anyone can take part in these projects by simply collecting data and following directions. They can also help identify interesting data points, unusual results, or other field data which they can push to researchers for further study in the lab. These kinds of interactions can connect excited and motivated citizen scientists to plant researchers and breeders in ways not previously possible. Ultimately we hope to build a non-hierarchical structure for anyone to ask and answer questions about our environment.
How the device works
for more information about all of the measurements and what they mean, please go here: http://blog.photosynq.org/what-you-can-measure-with-your-multispeq/
In a nutshell, the MultispeQ device is 2 photodiodes (aka really accurate light meters) and 8 LEDs, along with a bunch of sensors (temperature, relative humidity, CO2, light intensity, and color temperature (RGB)). The LEDs and detectors are located on opposite sides of the clamp, which means we can measure transmittance, reflectance, and fluorescence depending on which LEDs and which detector we use. If you only had the main device (no clamp), then you could measure reflectance and fluorescence. Different measurements use different wavelengths of light, so we have a variety installed, including 520, 605, 650, 730, 850, and 940 nm.
The device works by using pulse modulation. An example would be pulse modulated fluorescence. This method measures the amount of infra-red light (in the 680-700nm range) emitted by the chlorophyll in plant and algae cells in response to absorbing visible light. This infra-red ‘fluorescence’ relates directly to the amount of energy being absorbed by the plant to do useful work!
The problem is there are many similar infra-red light sources – like the sun, light bulbs, etc.. In order to separate the plant fluorescence from the other sources, we apply short pulses of visible light (25 millions of a second long) to the surface of the plant. The plant gives off pulses of infra-red light in response to the visible light pulses without perturbing the plant (very much). A detector is placed in front of the plant/algae to pick up the infra-red emissions. This detector only measures infra-red light (it doesn’t respond to visible light) and it uses AC filtering to pick up only the pulsed infra-red light from the plants, ignoring the constant infra-red sources coming from the sun and other stuff in the environment. This is pulse modulation.
The more advanced unit can also measure CO2, which can be used to measure soil biological activity.